Cytogenetic analysis of HACs created from unimolecular BAC–HAC vectors

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Rapid creation of BAC-based human artificial chromosome vectors by transposition with synthetic alpha-satellite arrays"</p><p>Nucleic Acids Research 2005;33(2):587-596.</p><p>Published online 26 Jan 2005</p><p>PMCID:PMC548352.</p><p>© The Author 2005. Published by Oxford University Press. All rights reserved</p> () Dual FISH/immunostaining with anti-CENP-C antibodies (red) and D17Z1 probe (green). () Two-color FISH analysis: D17Z1 probe (green) and 150 kb genomic fragment probe from BAC–HAC vector (red). () Two-color FISH analysis: D17Z1 probe (green) and BAC vector backbone probe (red). () Two-color FISH analysis: D17Z1 probe (green) and telomeric DNA (red). In all panels, DAPI-stained DNA in blue