The 5′-UTR of enhances translation when cap-dependent scanning is inhibited in a monocistronic construct

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Cryptic promoter activity in the DNA sequence corresponding to the 5′-UTR"</p><p>Nucleic Acids Research 2005;33(7):2248-2258.</p><p>Published online 20 Apr 2005</p><p>PMCID:PMC1083428.</p><p>© The Author 2005. Published by Oxford University Press. All rights reserved</p> () Schematic diagram of the constructs used. The 5′-UTR of was introduced immediately upstream of the luciferase open reading frame in the control plasmid pF to create pF-PIM. The stable hairpin structure with a free energy (Δ) of −55 kcal/mol was introduced upstream of the firefly luciferase start site in pF to create pHpF. The 5′-UTR of was introduced between the hairpin and the luciferase start site to create pHpF-PIM. () Relative luciferase activities of the monocistronic reporter constructs. Cos-7 cells were transfected with constructs pF, pF-PIM, pHpF and pHpF-PIM in combination with pSV-β-gal plasmid. Cell lysates were prepared 30 h post-transfection, and the activity of firefly luciferase was measured and normalized to that of β-galactosidase. The data were from four independent assays