Protein–protein interactions in the trypanosome U1 snRNP: TbU1-70K interacts with both TbU1C and TbU1-24K

Abstract

<p><b>Copyright information:</b></p><p>Taken from "U1 small nuclear RNP from : a minimal U1 snRNA with unusual protein components"</p><p>Nucleic Acids Research 2005;33(8):2493-2503.</p><p>Published online 29 Apr 2005</p><p>PMCID:PMC1087902.</p><p>© The Author 2005. Published by Oxford University Press. All rights reserved</p> () GST-fusion proteins of TbU1C, TbU1-24K and TbU1-70K, as well as GST alone as a control were immobilized on glutathione-Sepharose. Corresponding aliquots of immobilized proteins were analyzed for their protein content by SDS–PAGE and Coomassie staining. The arrows point to the proteins listed above the lanes. , protein marker (in kDa). () Immobilized GST proteins (as indicated above the lanes) were incubated with S-labeled TbU1C (lanes 1–4), TbU1-24K (lanes 5–8) or TbU1-70K (lanes 9–12). After washing, bound proteins were recovered and analyzed by SDS–PAGE and fluorography. The arrows point to the respective S-labeled proteins