Comparison of the substrate properties of 20/65 and 30/65 (3′-mismatched) duplexes

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Enzymatic processing of replication and recombination intermediates by the vaccinia virus DNA polymerase"</p><p>Nucleic Acids Research 2005;33(7):2259-2268.</p><p>Published online 20 Apr 2005</p><p>PMCID:PMC1083429.</p><p>© The Author 2005. Published by Oxford University Press. All rights reserved</p> Molecules containing 20mer or 30mer primer strands () were prepared as described in Materials and Methods, and incubated with vaccinia DNA polymerase in the presence of dNTPs. The 20mer strand and 30mer strand substrates are indicated. Also indicated are the 27mer, 25mer and 22mer bands that bear a 3′-terminal dCMP (‘C’, upper panel, at right)