Sections were multi-labeled with anti-ISG and anti-CD11b, anti-CD11c, anti-mPDCA1 or DAPI. A, ISG15 signals were observed in colonic lamina propria of R11567DA-treated mice while saline treatment produced no signal. B, C. Portions of ISG15cells (green) were co-stained (yellow) with anti-CD11b antibody (red). Nuclei were visualized by DAPI staining (blue) in Panel B. D, ISG15CD11cdouble positive cells (yellow) were scarcely found. E, In addition to ISG15CD11bdouble-positive cells (yellow), ISG15mPDCA1double positive cells (white) were found. However, more than half of ISG15cells was stained by neither anti-CD11b nor anti-mPDCA1 antibodies (data not shown).<p><b>Copyright information:</b></p><p>Taken from "Importance of the interferon-α system in murine large intestine indicated by microarray analysis of commensal bacteria-induced immunological changes"</p><p>http://www.biomedcentral.com/1471-2164/9/192</p><p>BMC Genomics 2008;9():192-192.</p><p>Published online 26 Apr 2008</p><p>PMCID:PMC2408602.</p><p></p
