The effects of the nucleotide compositions of the 3′-tail on displacement of partial heteroduplex substrates by Mcm4/6/7

Abstract

<p><b>Copyright information:</b></p><p>Taken from "DNA binding and helicase actions of mouse MCM4/6/7 helicase"</p><p>Nucleic Acids Research 2005;33(9):3033-3047.</p><p>Published online 25 May 2005</p><p>PMCID:PMC1140370.</p><p>© The Author 2005. Published by Oxford University Press. All rights reserved</p> () DNA helicase assays were performed with 3′-tailed partial heteroduplex helicase substrates (on a single-stranded circular DNA; 4 fmol) carrying various nucleotide sequences in the 3′-tail as shown. The asterisks represent the P-labeled 5′ ends of the annealed oligonucleotides. () DNA helicase assays were performed with similar sets of partial heteroduplex helicase substrates carrying the 3′-tails as shown. () Quantification of displaced oligonucleotides in (B). Lanes 1–4 contain 0, 25, 50 and 100 ng of the Mcm4/6/7 complex, respectively. B, boiled substrate