Mutational analysis of conserved positions comprising the C5–G57 base pair in the putative P1

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Core requirements for ribozyme self-cleavage reveal a putative pseudoknot structure"</p><p>Nucleic Acids Research 2006;34(3):968-975.</p><p>Published online 7 Feb 2006</p><p>PMCID:PMC1361622.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p>1 interaction. () Activity of six ribozyme construct designed to disrupt or maintain base pairing. Depicted is the proposed P1.1 interaction alone for each construct in the same orientation as shown in , where mutations relative to construct 1 are highlighted. Also shown are the GlcN6P-dependent self-cleavage activities of constructs 1 and 7–12 under standard conditions for 20 h as otherwise described in the legend to . Rate of self-cleavage in the presence of GlcN6P for constructs 1 (circles) and 7 (triangles) under standard conditions