Generation of functional HLA-DR*1101 tetramers receptive for loading with pathogen or tumour derived synthetic peptides-6

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Generation of functional HLA-DR*1101 tetramers receptive for loading with pathogen or tumour derived synthetic peptides"</p><p>BMC Immunology 2005;6():24-24.</p><p>Published online 5 Dec 2005</p><p>PMCID:PMC1325046.</p><p>Copyright © 2005 Moro et al; licensee BioMed Central Ltd.</p>roduction to different doses of p39 peptide. 10T cells are incubated for 48 hours with 5 × 10HLA-DR*1101LCL cells and the indicated doses of p39 peptide. IFN-γ in the supernatant is measured by ELISA. (b) Staining of a MAGE-3-specific T cell clone. The cells are stained at day 19 from re-stimulation with 10 μg of either CLIP-loaded or p39-loaded HLA-DR*1101-Bir tetramers for 2 hours at 37°C