Replacement of the essential Arp2 gene by its homologue using parasexual genetics-2

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Replacement of the essential Arp2 gene by its homologue using parasexual genetics"</p><p>http://www.biomedcentral.com/1471-2156/8/28</p><p>BMC Genetics 2007;8():28-28.</p><p>Published online 6 Jun 2007</p><p>PMCID:PMC1904233.</p><p></p>) Cell lysates prepared from equal numbers of cells were separated by SDS-PAGE, transferred onto nitrocellulose and immunoblotted as described in Materials and Methods. Blots were probed with anti-Arp2 antibody. (ii) 20 μg of total protein was separated by SDS-PAGE, transferred onto PVDF and immunoblotted as described in Materials and Methods. Blots were probed with anti-Arp2 and mAb 9E10 anti-antibodies. All results are representative two to three experiments. . Under-agar migratory response of cells to a gradient of folate. Arrows indicate the direction of cell migration (increasing folate concentration); Scale bar is 50 μm. Scale bar is 10 μm. Response of cells spotted on nitrocellulose filters, to a lateral light source (48 hrs). The light source is always to the right of the image. Arrows indicate the direction of migration; Scale bar is 5 mm. Scale bar is 1 mm. Fruiting body morphology of cells after development on nitrocellulose filters (48 hrs). Scale bar is 1 mm. Scale bar is 0.1 mm

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