() β-Galactosidase assay of the different loop–loop interactions investigated

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Stabilities of HIV-1 DIS type RNA loop–loop interactions and "</p><p>Nucleic Acids Research 2006;34(1):334-342.</p><p>Published online 12 Jan 2006</p><p>PMCID:PMC1331993.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> β-Galactosidase activity is expressed in Miller Units and was normalized relative to the activity of the XYLAI interaction. All hairpins were tested together with their cognate interaction partner (closed bars). As control all RNAs X and Y were assayed against non-cognate interaction partners, namely YΘ for RNAs X (light gray bars) and XΘ for RNAs Y (open bars). Furthermore transformants expressing only either XLAI-MS2 or m26-YLAI or no hairpin RNA at all are shown. s for the cognate kissing complexes at a concentration of 2 × 10 M are indicated. Error bars show the SD of two independent triplicate experiments. () Correlation between the and the β-galactosidase activity determined for kissing interactions containing 9 nt in the loop. (XYAA and YX0 contain 8 and 6 nt, respectively, and are not included.) () Schematic representation of cognate and non-cognate interactions considering XYLAI as example