Proapoptotic activity of Ukrain is based on L. alkaloids and mediated via a mitochondrial death pathway-12

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Proapoptotic activity of Ukrain is based on L. alkaloids and mediated via a mitochondrial death pathway"</p><p>BMC Cancer 2006;6():14-14.</p><p>Published online 17 Jan 2006</p><p>PMCID:PMC1379651.</p><p>Copyright © 2006 Habermehl et al; licensee BioMed Central Ltd.</p> extract adjusted to the respective chelidonine concentration. Apoptosis induction was analysed by fluorescence microscopy upon combined staining with Hoechst33342 and propidium iodide. The percentage of cells with characteristic apoptotic nuclear morphology was determined by counting a minimum of 250 cells per data point in each of at least three independent experiments. Data represent the percentage of cells with apoptotic nuclear morphology (means ± SD, n = 3). . Jurkat Vector cells were treated for 24 h with medium supplemented with solvent or 50 μM extract adjusted to the respective chelidonine-concentration. Apoptosis induction was analysed by fluorescence microscopy upon combined staining with Hoechst33342 and propidium iodide. Characteristic micrographs of nuclear morphology are shown. . Jurkat vector cells were treated for 24 h with medium supplemented with solvent or 1, 5, 10 and 50 μM L. extract adjusted to the respective chelidonine-concentration. Induction of apoptosis was then measured using flow cytometry (cell shrinkage: white bars; breakdown of mitochondrial membrane potential: grey bars; caspase-activation: black bars). Data show means ± SD (n ≥ 3)