() Dose–response determinations of the ·OH cleavage of DNA (black) and RNA (blue) following exposure of the nucleic acids to 5 mM Fe(II)-EDTA and 44 mM HO for the indicated period of time

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Fast Fenton footprinting: a laboratory-based method for the time-resolved analysis of DNA, RNA and proteins"</p><p>Nucleic Acids Research 2006;34(6):e48-e48.</p><p>Published online 31 Mar 2006</p><p>PMCID:PMC1421499.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> The data are best-fit by single exponentials with amplitudes and half-times of A = 0.54 & 0.44, A = 0.31 ± 0.02 & 0.30 ± 0.02 and t = 4.2 ± 0.9 and 6.1 ± 1.1 ms for DNA and RNA, respectively. The DNA dose–response curve was determined by quantification of the autoradiogram shown in . () The extent of ·OH mediated cleavage of DNA (black) and RNA (blue) determined at 44 mM HO with a 5 ms reaction time as a function of Fe(II)-EDTA concentration. The solid lines are fits of the data to a simple saturation function. Open and closed symbols are replicate experiments in both (A) and (B)