Optimal reaction conditions for the 3′–5′ exonuclease activity of the Ape2

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Human Ape2 protein has a 3′–5′ exonuclease activity that acts preferentially on mismatched base pairs"</p><p>Nucleic Acids Research 2006;34(9):2508-2515.</p><p>Published online 10 May 2006</p><p>PMCID:PMC1459411.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> The 3′–5′ exonuclease activity of GST–Ape2 was assayed under various reaction conditions using a partial DNA duplex (S3) (10 nM) in which the 5′-labeled oligonucleotide contained a 3′-recessed terminus. () Metal ion dependence. The first lane contains reaction mixture without any metal ions in the reaction buffer. Reactions were carried in the presence of 8 mM MgCl (lanes 2–4) or 0.5 mM MnCl (lanes 5–7) and increasing concentrations of Ape2 as indicated. () Graphical representation of results in (A); () NaCl concentration dependence. The first lane contains reaction without any Ape2 protein. () Graphical representation of results in (C); () pH dependence. () Graphical representation of results in (E)