Selection, affinity maturation, and characterization of a human scFv antibody against CEA protein-1

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Selection, affinity maturation, and characterization of a human scFv antibody against CEA protein"</p><p>BMC Cancer 2006;6():41-41.</p><p>Published online 24 Feb 2006</p><p>PMCID:PMC1402309.</p><p>Copyright © 2006 Pavoni et al; licensee BioMed Central Ltd.</p>bated with irrelevant anti-glucose oxidase (a protein not present in mammals) scFv antibody (anti-G.O., right slide). B. Biochemical patterns of the MA39 on purified CEA (lane 1) and LoVo cellular extract (lane 2). Lanes 3 and 4 show the reactivity of identical proteins with irrelevant anti-G.O. scFv. C. Staining of cryosections from human colon carcinoma xenograft in mouse. Commercially available anti-CEA antibody and irrelevant anti-G.O. antibodies were used as positive and negative controls, respectively