Selection, affinity maturation, and characterization of a human scFv antibody against CEA protein-4

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Selection, affinity maturation, and characterization of a human scFv antibody against CEA protein"</p><p>BMC Cancer 2006;6():41-41.</p><p>Published online 24 Feb 2006</p><p>PMCID:PMC1402309.</p><p>Copyright © 2006 Pavoni et al; licensee BioMed Central Ltd.</p>s. Significant fluorescence shift indicates difference in affinity of two single-chain antibodies. B. Western blot with serial dilutions of CEA protein was stained by using original MA39 and maturated E8 scFv antibodies. The membranes were developed with secondary anti-FLAG AP-conjugated antibody