Competition of the 13 bp substrate duplexes and the Tg-containing duplexes in the Endo III reaction

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Synthesis and characterization of oligonucleotides containing 2′-fluorinated thymidine glycol as inhibitors of the endonuclease III reaction"</p><p>Nucleic Acids Research 2006;34(5):1540-1551.</p><p>Published online 17 Mar 2006</p><p>PMCID:PMC1409675.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> The P-labeled substrates without fluorine were incubated with Endo III in the presence of the competitors containing Tg. The amounts of the nicked products (standardized to those without the competitors) were plotted against the concentrations of the competitor. Open circles, P-5-Tg + 5-Tg; filled circles, P-5-Tg + 5-Tg; open triangles, P-5-Tg + 5-Tg; filled triangles, P-5-Tg 5-Tg