A truncated Kv1.1 protein in the brain of the mouse: expression and interaction-0

Abstract

<p><b>Copyright information:</b></p><p>Taken from "A truncated Kv1.1 protein in the brain of the mouse: expression and interaction"</p><p>BMC Neuroscience 2005;6():65-65.</p><p>Published online 23 Nov 2005</p><p>PMCID:PMC1322225.</p><p>Copyright © 2005 Persson et al; licensee BioMed Central Ltd.</p> SDS-PAGE and immunoblotted with the polyclonal Kv1.1 N-terminal antibody. In wild type lysate a strong band was detected at 86 kDa, corresponding to Kv1.1. The same band was seen in the Kv1.1 null and lysates but at a lower intensity. The 86 kDa bands in Kv1.1 null and lysates are due to antibody cross reactivity since neither Kv1.1 null nor mice have any full-length Kv1.1 protein. B. The polyclonal Kv1.1 N-terminal antibody was preincubated with the peptide used for immunization. Lysate from wild type (+/+) and (m/m) brains were loaded on SDS-PAGE and immunoblotted with the Kv1.1 N-terminal antibody without or after preincubation. The preincubation completely blocked the signal. C. A longer exposure of the immunoblot in panel A. In brain lysate there was a unique band at approximately 30 kDa, which corresponds to the calculated weight of MCEPH (arrow)