Correlation between the dose of cisplatin (exposure: 2 h; 37°C) and the level of specific intrastrand crosslinks in the nuclear DNA of cultivated human Jurkat T cells

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Adduct-specific monoclonal antibodies for the measurement of cisplatin-induced DNA lesions in individual cell nuclei"</p><p>Nucleic Acids Research 2006;34(6):e47-e47.</p><p>Published online 29 Mar 2006</p><p>PMCID:PMC1420801.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> Adduct levels were measured after immunostaining with anti-(Pt-DNA) Mab R-C18 (Pt-[GG] lesions (), or R-B3 specific for Pt-(AG) () and quantitative ICA image analysis (Materials and Methods). Antibody-derived signals from individual nuclei were normalized for the actual DNA content of each cell. Values are given as AFU and represent means ± SE of 200 analyzed cells per dose. Correlation: (A) = 0.96; (B) = 0.91