Substrates 1HU (left column) and 2HU (right column) were mixed with increasing concentrations of D88N/H210N UNG using stopped-flow

Abstract

<p><b>Copyright information:</b></p><p>Taken from "A rapid reaction analysis of uracil DNA glycosylase indicates an active mechanism of base flipping"</p><p></p><p>Nucleic Acids Research 2007;35(5):1478-1487.</p><p>Published online 6 Feb 2007</p><p>PMCID:PMC1865060.</p><p>© 2007 The Author(s).</p> Anisotropy ( and ) and total HEX fluorescence ( and ) were simultaneously monitored, and the same solutions were then used to collect 2-AP fluorescence ( and ). The data are shown with the results of a global fit to Scheme 1. Individual curves for each of the enzyme concentrations used are shown: 8 μM (red); 3 μM (green); 2 μM (blue); 1 μM (cyan); 0.5 μM (magenta) and 0.2 μM (purple), all reactions were performed with 0.1 μM substrate and other conditions as described in the Materials and methods section