Rates of chemical cleavage were determined by mixing UNG and either substrate 3U () or 4U ()

Abstract

<p><b>Copyright information:</b></p><p>Taken from "A rapid reaction analysis of uracil DNA glycosylase indicates an active mechanism of base flipping"</p><p></p><p>Nucleic Acids Research 2007;35(5):1478-1487.</p><p>Published online 6 Feb 2007</p><p>PMCID:PMC1865060.</p><p>© 2007 The Author(s).</p> The observed rates () are plotted against enzyme concentration. ( Data for the AT-rich single-stranded oligonucleotide 3U is shown with the best fit to Equation (), with values of = 37.5 ± 1.8 s and = 3.9 ± 0.5 μM. () The data for the GC-rich single-stranded oligonucleotide 4U did not reach saturation and exhibited a linear rather than hyperbolic relationship, hence is shown with the best fit to a linear equation