Analysis of the reduction of TurboGFP levels by antisense mimic oligomers in Phoenix Eco cells transfected with p2FP-RNAi vector

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Hydroxyproline-based DNA mimics provide an efficient gene silencing and "</p><p>Nucleic Acids Research 2006;34(8):2247-2257.</p><p>Published online 2 May 2006</p><p>PMCID:PMC1456331.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> Co-transfection of oligomers was performed in the presence of LFA. The cells transformed with the vector alone were analyzed as controls. () Sequences of oligomers designed to target the translational start site of the TurboGFP mRNA. () Analysis of green and red fluorescence intensity of the cells treated with 0.5 µM oligomers, or with 0.2 µM dsRNA, after 24 h. The average data of three separate experiments are shown. () Fluorescent microscopy images of the cells transfected with p2FP-RNAi and treated with 0.5 µM mimic oligomer samples for 16 and 24 h. Panels show examples of cells treated with 0.2 µM dsRNA (a); mismatched pHypNA oligomer (b); duplex of antisense pHypNA oligomer with CT-ODN (c); duplex of antisense HypNA-pPNA oligomer with CT-ODN (d); antisense pHypNA oligomer (e) and antisense HypNA-pPNA oligomer (f). Panel (g) shows cells untreated with oligomers. () Effect of the antisense oligomer concentration on the TurboGFP production in cells. Fluorescence was measured 24 h after the transfection. Average data points from three independent experiments are shown