Potentiality of Mixed Cellulolytic Microbes of Termite Extract, Elephant Feces Solution and Buffalo Rumen Fluid

Abstract

This research consisted of two experiments. The first experiment was conducted to get cellulolytic microbes of termite extract (RE), elephant feces solution (GLF), buffalo (KCR) and cattle (SCR) rumen fluid. Twelve hungate tubes each contained of 13,5 ml limited media [(NH4)2SO4, CaCO3, K2HPO4, MgSO4.7H2O, NaCl, paper filter, aquadest] was divided to four treatments based on microbe resources (MR), namely: RT (MR: RE), GT (MR: GLF), KT (MR: KCR), and ST (MR: SCR). The average of specific activity change of CMCace KT (0.0753 + 0.0170 U/mg) treatment was higher and significantly different (P<0.05) than those of RT (- 0.0677 + 0.0099), GT (0.0229 + 0.0034 U/mg), and ST (0.0145 + 0.0074 U/mg) treatment. The second experiment was aimed to know potentiality of cellulolytic microbes from the first experiment and their several combinations in degrading cellulose. Twenty four hungate tubes each contained of 10 ml growth media [(NH4)2SO4, MgSO4.7H2O, NaCl, CaCl2.2H2O, K2HPO4, KH2PO4, cellulose, resazurine, yeast, cycteine- HCl, and aquadest] was divided to eight treatments based on microbe resources (MR), namely: RP (MR: RT),GP (MR: GT), KP (MR: KT), SP (MR: ST), RGP (MR: RT+GT), RKP (MR: RT+KT), RSP (MR: RT+ST), RGKP (MR: RT+GT+KT).). The average of specific activity change of CMC-ase RGKP (0.2344 + 0.0014 U/mg) treatment was higher and significantly different (P<0.01) than those of RP (0.0781 + 0.0015 U/mg), GP (0.0060 + 0.0006 U/mg), KP (0.0117 + 0.0005 U/mg), SP (0.0285 + 0.0009 U/mg), RGP (0.0569 + 0.0014 U/ mg), RKP (0.0965 + 0.0023 U/mg), and RSP (0.0868 + 0.0032 U/mg). Potentiality of mixed cellulolytic microbes containing termite extract, elephant feces solution, and buffalo rumen fluid (RGKP treatment) in degrading cellulose was the highest. Keywords: Cellulolytic Microbes, Termite Extract, Elephant Feces Solution, Buffalo Rumen Flui