Inhibition of translation by the uORF is independent of its peptide sequence and )

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Translational repression of mouse mu opioid receptor expression via leaky scanning"</p><p></p><p>Nucleic Acids Research 2007;35(5):1501-1513.</p><p>Published online 6 Feb 2007</p><p>PMCID:PMC1865057.</p><p>© 2007 The Author(s).</p> Schematic representations of the DNA constructs used in this study. Shaded triangles indicate point mutations to the Stop (S) codon; vertical dotted lines indicate point mutations of ATG to ACG (M) ( and ). Forty-eight hours after transfection, cells were trypsinized and examined for luciferase and ß-galactosidase activities, as well as by RNA extraction and transcript quantification. Relative LUC activity and mRNA levels were expressed as the ratio LUC/ß-gal and LUC/LacZ, as described in Materials and Methods