Effect of the expression of the polβ and polμ inactive form on NHEJ events

Abstract

<p><b>Copyright information:</b></p><p>Taken from "The DNA polymerase λ is required for the repair of non-compatible DNA double strand breaks by NHEJ in mammalian cells"</p><p>Nucleic Acids Research 2006;34(10):2998-3007.</p><p>Published online 31 May 2006</p><p>PMCID:PMC1474058.</p><p>© 2006 The Author(s)</p> ( and ) Representation of the sequences of the I-Sce-I restriction sites in the C′10 and A′7H cell lines, respectively. In the C′10 cells the two I-Sce-I sites are in direct orientation whereas in the A′7H cells the two I-Sce-I sites are in inverted orientation (upper panel). Evaluation of the frequency of the deletion (CD4) and inversion (CD8) events in the different cell lines (median panel). Results are the mean +/− SD of three independent experiments. Western blots for polβ and polμ expression in the different cell lines (lower panel)