The expression levels from each respective initiation codon within the mouse MOR uORF region

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Translational repression of mouse mu opioid receptor expression via leaky scanning"</p><p></p><p>Nucleic Acids Research 2007;35(5):1501-1513.</p><p>Published online 6 Feb 2007</p><p>PMCID:PMC1865057.</p><p>© 2007 The Author(s).</p> () A schematic summary of various in-frame constructs. Mouse MOR uORF regions were fused in-frame to the luciferase gene. Initiation codons with vertical dotted lines indicate point mutations of ATG to ACG. () Autoradiogram of the proteins translated by a coupled transcription/translation system in the presence of [S]-methionine from representative translations. Proteins were separated by 10% SDS-PAGE (LUC, Fused Protein). Quantification of mRNA levels from the cell-free transcription/translation system was performed by RT-PCR (+/− RT). Expression levels from each initiation codon were normalized against those of SP6-LUC. Normalized mRNA levels (lower panel). M: 100 bp molecular weight markers. () Relative strength of each initiation context within the mouse MOR UTR as determined by translation. The LUC ATG context is designated 1.00