Effect of L20 overproduction on the transcription profile of the operon

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Ribosomal protein L20 controls expression of the operon via a transcription attenuation mechanism"</p><p></p><p>Nucleic Acids Research 2007;35(5):1578-1588.</p><p>Published online 8 Feb 2007</p><p>PMCID:PMC1865079.</p><p>© 2007 The Author(s).</p> Northern analysis of total RNA of a wild-type strain harboring plasmid pHML17 carrying an IPTG inducible copy of the (L20) gene was performed using two different probes. () RNA was separated on a 0.8% agarose gel and probed with an specific probe including the entire leader sequence (see Materials and methodssection). () RNA separated on a 8% polyacrylamide gel was probed with oligonucleotide HP1080 complementary to positions +8 to +34 of the leader. Where indicated IPTG (1 mM) was added to mid-log cultures for 20 min prior to isolation of the RNA. RT = read-through transcript, T = terminated transcript. The positions of the molecular size markers are indicated. Percentages of read-through transcripts are indicated below the gel