<p><b>Copyright information:</b></p><p>Taken from "Molecular Mechanisms of the Diabetogenic Effects of Arsenic: Inhibition of Insulin Signaling by Arsenite and Methylarsonous Acid"</p><p></p><p>Environmental Health Perspectives 2007;115(5):734-742.</p><p>Published online 29 Jan 2007</p><p>PMCID:PMC1867998.</p><p>This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original DOI</p> () Immunoblot analyses of the activated PI-3K, total PTEN, and phosphorylated PTEN (Ser380) in control 3T3-L1 adipocytes before or after activation with insulin and in insulin-activated adipocytes treated for 4 hr with 50 μM iAs or 2 μM MAs. Activated PI-3K was immunoprecipitated from control and exposed cells with an anti-phospho-Tyr (PY20) antibody and immunoblotted with an antibody against the regulatory (p85) subunit. Representative blots of three independent experiments are shown. () The ratio of phosphorylated PTEN (Ser380) to total PTEN expressed as a percent of the ratio found in control adipocytes before activation with insulin. Each value represents the mean ± SD; = 3 experiments
