Formation of the covalent complex between T4 Endo V and a CPD-containing oligonucleotide duplex fluorinated at the 2′ position

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Synthesis and characterization of oligonucleotides containing 2′-fluorinated thymidine glycol as inhibitors of the endonuclease III reaction"</p><p>Nucleic Acids Research 2006;34(5):1540-1551.</p><p>Published online 17 Mar 2006</p><p>PMCID:PMC1409675.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> The 5′ and 3′ ends of the CPD-containing strand were P-labeled. After hybridization with the complementary strand, the duplexes were incubated with T4 Endo V at 30°C for 30 min, and the mixtures were subjected to 15% SDS–PAGE