Example of an extended UTR bridging the gap to a distant full-length cDNA

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Beyond the 3′ end: experimental validation of extended transcript isoforms"</p><p></p><p>Nucleic Acids Research 2007;35(6):1947-1957.</p><p>Published online 4 Mar 2007</p><p>PMCID:PMC1874610.</p><p>© 2007 The Author(s)</p> Screenshot from the Riken genomic element viewer () displaying both full-length cDNAs and CAGE tags (transcription starts) for gene Tmem33. The stop codon position is indicated with a yellow triangle. The bottom red strip represents the RT-PCR validated transcript (5′ end is not determined). We located a putative poly(A) site for gene Tmem33 at around 5.2 kb from the stop codon, supported by a single full-length cDNA (highlighted). The 2.35 kb gap between this unique cDNA and the longest Tmem33 transcript caused Riken to predict two independent transcription units (TUs) for these entities