HMGA2-mediated transcriptional activation is dependent upon basic residues within the second AT-hook

Abstract

<p><b>Copyright information:</b></p><p>Taken from "The second AT-hook of the architectural transcription factor HMGA2 is determinant for nuclear localization and function"</p><p></p><p>Nucleic Acids Research 2007;35(6):1751-1760.</p><p>Published online 25 Feb 2007</p><p>PMCID:PMC1874589.</p><p>© 2007 The Author(s)</p> () Diagram of luciferase reporter gene under the transcriptional regulation of the cyclin A promoter and the HMGA2 wild type and mutants expressed by the vectors used. () CHO cells were transiently cotransfected with 1 µg of the CycA luciferase reporter plasmid (bars 1–4) and with 3 µg of HMGA2 wt-, mS5- and mT6- EGFP expression vectors (bars 2–4 respectively). 0.1 µg of pRL-CMV Renilla luciferase expression vector was included to normalize for transfection efficiencies. Values are reported as relative luciferase activity. Standard deviations are indicated for experiments repeated three times. The amount of HMGA2 wt-, mS5- and mT6-EGFP expression was assayed by Western blot analysis using a polyclonal α-HMGA2 antibody. The subcellular localization of the expressed proteins in CHO cells was confirmed by confocal microscopy (data not shown)