<p><b>Copyright information:</b></p><p>Taken from "Genomic analysis of the secretion stress response in the enzyme-producing cell factory "</p><p>BMC Genomics 2007;8():158-158.</p><p>Published online 11 Jun 2007</p><p>PMCID:PMC1894978.</p><p></p> Fractions are numbered from the top to the bottom of the gradient. (B) RNA was extracted from each fraction and subjected to electrophoresis through a formaldehyde gel. The ribosomal RNA distribution profile (25S, 18S and 5S rRNA; indicated by arrowheads) enables the the assignment of ODpeaks, corresponding to the 40S and 60S ribosomal subunits and to intact ribosomes (80S). (C) RT-PCR analysis with primers were performed from each fraction of collected gradients from treated and non-treated cells. The full length mRNA (arrow) as well as low-molecular-weight version of (arrowhead) can be visualized
