Model for dynamic binding of Ku80, Ku70, NF90, NF45 and DNA-PKcs to the IL-2 proximal promoter in resting and activated T-cells

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Dynamic binding of Ku80, Ku70 and NF90 to the IL-2 promoter in activated T-cells"</p><p></p><p>Nucleic Acids Research 2007;35(7):2302-2310.</p><p>Published online 27 Mar 2007</p><p>PMCID:PMC1874627.</p><p>© 2007 The Author(s)</p> () In resting T-cells, Ku70 and Ku80 bind to the ARRE sequence in the IL-2 proximal promoter and contribute to a closed chromatin conformation and transcriptional repression. () T-cell activation induces chromatin remodeling associated with decreased binding of Ku70 to the IL-2 promoter and increased binding of NF90 and Ku80 to the IL-2 promoter. Associations between NF45/NF90 heterodimer and Ku70. Ku80 and DNA-PKcs are weakened. IL-2 chromatin remodeling is linked to the conversion of the purine-box regulator from a transcriptional repressor into a potent transcriptional activator, indicated by new interactions of transactivation (TA) domains on NF90 and NF45 with RNA Pol II