Mapping the survivin promoter region that mediates Hoechst33342's effects on the induction of survivin promoter activity

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Molecular mechanism of upregulation of survivin transcription by the AT-rich DNA-binding ligand, Hoechst33342: evidence for survivin involvement in drug resistance"</p><p></p><p>Nucleic Acids Research 2007;35(7):2390-2402.</p><p>Published online 28 Mar 2007</p><p>PMCID:PMC1874665.</p><p>© 2007 The Author(s)</p> () The 781-bp DNA region between pLuc-1430 and pLuc-649 was identified to mediate the upregulation of survivin promoter activity by Hoechst33342. HeLa cells were transfected with various survivin promoter-luciferase constructs as shown and treated with or without Hoechst33342 (5 μM) 24 h after transfection. Cells were lysed and luciferase activities were determined 24 h after treatment. () Nested deletion of the 781-bp DNA region between pLuc-1430 and pLuc-649 identified a 117-bp DNA region mediating a major effect of Hoechst33342. Transfection, drug treatment and luciferase assay are as in (A). In both (A) and (B), luciferase activities were normalized to Renilla luciferase internal controls as arbitrary units and are shown as a histogram. Each bar is the mean ± SD from the experiment in triplicate