Electrophoretic mobility shift analyses for the determination of the binding affinity of the wt R

Abstract

<p><b>Copyright information:</b></p><p>Taken from "R.KpnI, an HNH superfamily REase, exhibits differential discrimination at non-canonical sequences in the presence of Ca and Mg"</p><p></p><p>Nucleic Acids Research 2007;35(8):2777-2786.</p><p>Published online 11 Apr 2007</p><p>PMCID:PMC1885652.</p><p>© 2007 The Author(s)</p>KpnI and mutant H149A in the presence of Ca. () and () Gel shift showing the native gel (inset) with duplex II and III. The wt R.KpnI was titrated over the concentration range from 0 to 256 nM, and the concentration of the DNA was 25 pM. The intensity of the shifted DNA band are expressed as% bound. (), () and () are the gel shifts showing the native gels (inset) with duplex I, II and III respectively using mutant H149A. The average values of the three different experiments were plotted in the graph