The effects of CHX and LMB on GSNO-induced cytosolic translocation of APE1

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Nitric oxide controls nuclear export of APE1/Ref-1 through S-nitrosation of Cysteines 93 and 310"</p><p></p><p>Nucleic Acids Research 2007;35(8):2522-2532.</p><p>Published online 1 Apr 2007</p><p>PMCID:PMC1885639.</p><p>© 2007 The Author(s)</p> () Effect of CHX. 30 h after transfection, cells were treated with 1 mM GSNO for 4 h in the presence or absence of CHX, and then fixed and immunostained by HA antibody and a Texas-red labeled secondary antibody, counterstained with Hoechst 33342, and analyzed by fluorescence microscopy. () Effect of LMB. HEK293 cells transfected with HA-APE1 were treated with 5 ng/ml LMB for 4 h, followed by 1 mM GSNO for another 4 h, and then the subcellular distribution of HA-APE1 was determined. () Positive control of the effect of LMB on the localization of GFP-IκB expressed in HEK293 cells