<p><b>Copyright information:</b></p><p>Taken from "A first step in understanding an invasive weed through its genes: an EST analysis of invasive "</p><p>http://www.biomedcentral.com/1471-2229/7/25</p><p>BMC Plant Biology 2007;7():25-25.</p><p>Published online 24 May 2007</p><p>PMCID:PMC1890287.</p><p></p> of . Five thousand ESTs were sequenced from the 5' end (Agencourt biosciences), and assembled into 4,423 contigs, or 'unigenes.' Unigenes were translated in all frames and the resulting amino acid sequences were used as BLAST queries. Top BLAST hits provided annotation and functional categorization (gene ontology assignment) for each unigene. Not all unigenes were able to be annotated by GO programs. Computational analysis was done using the PLAN database (Noble Foundation)