Cleavage of 5′-P-labelled RNA-96 by and RNase T1 under various conditions

Abstract

<p><b>Copyright information:</b></p><p>Taken from "RNase T1 mimicking artificial ribonuclease"</p><p></p><p>Nucleic Acids Research 2007;35(7):2356-2367.</p><p>Published online 27 Mar 2007</p><p>PMCID:PMC1874650.</p><p>© 2007 The Author(s)</p> Autoradiograph of 12% polyacrylamide–8 M urea gel. Lanes L—imidazole ladder. Lanes C—RNA incubated in the absence of cleaving agents for 8 h and 15 min, respectively. reaction: [5′-P]-RNA-96 was incubated in the presence of 10 μM in buffers and at 37°C for 5–8 h. T1-reaction: [5′-P]-RNA-96 was incubated in the presence of 3–5 U RNase T1 in buffer at 50°C and in buffers or at 37°C for 15 min. Buffers are listed in the Materials and methods section. Incubation time, concentration of RNase T1 and experimental conditions are indicated in the top. Location of cleavage sites is shown on the left and right