To load lysosomes HeLa (A, B and D), or C2BBe1 (C), cells were preincubated with dextran-647 (red) o/n, then chased in dextran-free media for 3 h before infection with Red- (blue)

Abstract

<p><b>Copyright information:</b></p><p>Taken from " Trafficking is Defined by Continuous Dynamic Interactions with the Endolysosomal System"</p><p></p><p>Traffic (Copenhagen, Denmark) 2007;8(3):212-225.</p><p>Published online 15 Jan 2007</p><p>PMCID:PMC2063589.</p><p>© 2007 The Authors Journal compilation © 2007 Blackwell Publishing Ltd</p> After infection, cells were incubated with dextran-488 (green) to load endosomes and live-cell imaging was initiated at 3 h p.i. A) A single confocal plane showing co-localization of dextran-647 and dextran-488 in a single SCV (arrow). B) A projection of 36 focal planes with YZ (red line) and XZ (blue line) side views to show co-localization of dextrans. C) A single confocal plane from the center of Red--infected C2BBe1 epithelial cells, showing co-localization of dextran-647 and dextran-488 in individual SCVs (arrows). D) Selected frames from a time-lapse series showing delivery of dextran-488 (top) from preloaded lysosomes to an SCV (arrows). Image acquisition started 15 min p.i. (00:00). Scale bars = 5 μm