Total RNA isolated at 6 h post infection of HeLa cells with indicated viruses was analyzed by RNA protection assay using RiboQuant Multi-Probe template set hCK-5
<p><b>Copyright information:</b></p><p>Taken from "Porcine adenovirus type 3 E1Bprotein downregulates the induction of IL-8"</p><p>http://www.virologyj.com/content/4/1/60</p><p>Virology Journal 2007;4():60-60.</p><p>Published online 12 Jun 2007</p><p>PMCID:PMC1906745.</p><p></p> The protected band indicated by the label on the right migrate faster that undigested probes, as expected.HeLa cells transfected with the human IL-8 promoter containing a NF-κB recognition sequence, cloned upstream from a luciferase reporter cDNA in the presence of plasmid pCDNA3.1 or pCDNA3.1-pE1BL were assayed for luciferase activity (expressed as relative light units [RLU]). The error bars represent the standard error of mean of triplicate samples
