Subtle differences in CD8α between monocytes and lymphocytes detected by 2-D electrophoresis

Abstract

<p><b>Copyright information:</b></p><p>Taken from "CD8α is expressed by human monocytes and enhances FcγR-dependent responses"</p><p>http://www.biomedcentral.com/1471-2172/8/12</p><p>BMC Immunology 2007;8():12-12.</p><p>Published online 1 Aug 2007</p><p>PMCID:PMC2000912.</p><p></p> , Western blot with a polyclonal anti-CD8α antibody after 2-D electrophoresis of monocytes and lymphocytes. Cell lysates of lymphocytes and monocytes from one donor were separated by adherence, halved and treated (bottom panels) or not treated (top panels) with neuraminidase before analysis. , Western blot with anti-CD8α mAb D9. , Neuraminidase treatment and western blot with D9 of lymphocyte lysate. Results are representative of experiments with three donors