<p><b>Copyright information:</b></p><p>Taken from "Functional distribution of Ca-coupled P2 purinergic receptors among adrenergic and noradrenergic bovine adrenal chromaffin cells"</p><p>http://www.biomedcentral.com/1471-2202/8/39</p><p>BMC Neuroscience 2007;8():39-39.</p><p>Published online 14 Jun 2007</p><p>PMCID:PMC1906789.</p><p></p>acellular calcium (B) and during stimulation in the virtual absence of extracellular calcium (C, "UTP+EGTA"). At the end of each experiment cells were stimulated with 10 μM nicotine. Cells were allowed to rest for 10 min between consecutive stimulations. The fura-2 fluorescence ratio F/Fwas determined for each cell in a field on a pixel-by-pixel basis. Images were coded in pseudocolor to show differences in the F/Fratio. The images corresponding to UTP stimulation (B and C) were captured ~5 s after the UTP challenges (i.e. at the response peak in presence of extracellular calcium); . Pseudocolor image of PNMT/TH immunofluorescence ratio (labeling index) and corresponding scale. Intensely colored red and green cells are adrenergic and noradrenergic cells, respectively. Scale bar = 50 μm; . Time courses of changes in F/Ffluorescence ratio for two AD-cells (cells 1 and 2, also depicted in A-D) and two NA-cells (cells 3 and 4). The lines denote superfusions with UTP or nicotine in the presence or virtual absence (middle traces) of extracellular calcium
