Accumulation kinetics of a series of deletion fragments with or without F6A/F7A substitution

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Recruitment of DNA repair synthesis machinery to sites of DNA damage/repair in living human cells"</p><p></p><p>Nucleic Acids Research 2007;35(9):2913-2923.</p><p>Published online 16 Apr 2007</p><p>PMCID:PMC1888830.</p><p>© 2007 The Author(s)</p> () Sequence alignment of the N-terminal portion of RFC140 orthologs from nine eukaryotes. Approximately 10 amino-acid residues are indicated. Red-colored residues are highly conserved among all species, and are indicated as the PIP-box (see text). EGFP-fused fragments 1–369 (), 1–397 (), 1–493 (), 1–733 () and full-length (1–1147, ) having normal (closed squares) or the F6A/F7A mutation (open squares) were transiently expressed in HeLa cells and accumulation was measured as the fold increase of fluorescence intensity. Error bars indicate standard error. Error bars not indicated are smaller than symbols. Pictures represent accumulation of FA mutant fragments, and are taken before and 120 min after laser irradiation. Data were taken from five independent experiments. White arrowheads indicate the laser irradiation region