<p><b>Copyright information:</b></p><p>Taken from "Adoptively transferred human lung tumor specific cytotoxic T cells can control autologous tumor growth and shape tumor phenotype in a SCID mouse xenograft model"</p><p>http://www.translational-medicine.com/content/5/1/29</p><p>Journal of Translational Medicine 2007;5():29-29.</p><p>Published online 25 Jun 2007</p><p>PMCID:PMC1933531.</p><p></p> Mice were injected with 5 × 10tumor cells intravenously. On day 5, after tumor foci were established in the lungs, 5 × 10CTLs were injected intravenously. On day 10, mice were sacrificed and lung and liver were excised for immunohistochemical and PCR analysis. and . IHC analysis of lungs for the presence of CD8 positive CTLs. Lungs from mice injected with . tumor cells + tumor antigen-specific CTLs . tumor cells only, . tumor cells + influenza nucleoprotein antigen-specific CTL or . tumor antigen-specific CTL alone. Original magnification: ×200, brown staining shows CD8 positive T cells. and . RT-PCR analysis. Primers used are specific for human . CD8, . IFN-γ and . β-actin. , 100 bp DNA ladder; , cDNA from lung of SCID mice bearing human tumor only; , cDNA from liver of SCID mice bearing with tumor only; , cDNA from lung of SCID mice bearing human tumor and CTLs; , cDNA from liver of SCID mice bearing tumor and CTLs
