Repressor of temperate mycobacteriophage L1 harbors a stable C-terminal domain and binds to different asymmetric operator DNAs with variable affinity-3
<p><b>Copyright information:</b></p><p>Taken from "Repressor of temperate mycobacteriophage L1 harbors a stable C-terminal domain and binds to different asymmetric operator DNAs with variable affinity"</p><p>http://www.virologyj.com/content/4/1/64</p><p>Virology Journal 2007;4():64-64.</p><p>Published online 28 Jun 2007</p><p>PMCID:PMC1934351.</p><p></p>estimated from inset gel shift assay pictures) versus CI concentration (0.05 – 0.45 μM and 0.1 – 0.8 μM CI with and , respectively) are shown. Nearly 0.1 nM labeled operator was used in each reaction. Plot of %operator bound versus time shows the kinetics of CI dissociation from and operators in presences of excess cold operator. The amount of operator bound in the shifted complex of the zero time aliquot was considered as 100%. Plot of log Keq versus 1/T shows equilibrium binding of CI to operator at temperatures ranging from 25° – 42°C. All curves/lines are best-fit curves/lines
