Replacement of the essential Arp2 gene by its homologue using parasexual genetics-3

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Replacement of the essential Arp2 gene by its homologue using parasexual genetics"</p><p>http://www.biomedcentral.com/1471-2156/8/28</p><p>BMC Genetics 2007;8():28-28.</p><p>Published online 6 Jun 2007</p><p>PMCID:PMC1904233.</p><p></p>alysed by SDS-PAGE and western blotting as described in Materials and Methods. Cell lysates (L) and immunoprecipitates (IP) from three cell types were examined; AX3 (wild type), MZ10 (haploid rescued with -tagged Arp2 replacement), MZ11 (haploid rescued with -tagged Arp2 replacement). Blots were probed with antibodies to Arp2, Arp3 and the p21-arc subunits