Biochemical characterization of a recombinant Japanese encephalitis virus RNA-dependent RNA polymerase-7

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Biochemical characterization of a recombinant Japanese encephalitis virus RNA-dependent RNA polymerase"</p><p>http://www.biomedcentral.com/1471-2199/8/59</p><p>BMC Molecular Biology 2007;8():59-59.</p><p>Published online 11 Jul 2007</p><p>PMCID:PMC1934914.</p><p></p>hown below the structures predicted by the Mfold program. Partial structures representing the lower stem-region are shown. The deleted and added nucleotides are indicated by ρ and boxed, respectively. (B) RNA synthesis directed by the wild-type 83-nt RNA and its derivatives. RdRp assays were performed with the RNA templates indicated above the autoradiogram, and products were resolved a 5% polyacrylamide sequencing gel (20 × 40 cm) containing 8 M urea. Arrowhead indicates the 81-nt internally initiated RNA product. Representative data from three independent experiments are shown

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