<p><b>Copyright information:</b></p><p>Taken from "Domain selection combined with improved cloning strategy for high throughput expression of higher eukaryotic proteins"</p><p>http://www.biomedcentral.com/1472-6750/7/45</p><p>BMC Biotechnology 2007;7():45-45.</p><p>Published online 30 Jul 2007</p><p>PMCID:PMC1950093.</p><p></p>DS and BatchPrimer were two PERL programs used for extraction of the DNA coding sequence from a full-length sequence (ORF) and design of gene specific primers