<p>Immunofluorescence staining of A) total endogenous CAR (green) and B) CAR<sup>Ex8</sup> (green), co-stained with antibodies directed against either the tight junction protein ZO-1 (red) or the apical protein ezrin (red), in polarized CaLu-3 cells 18 h after exposure to SSFA or SSS. Nuclei are counterstained with DAPI (blue). X–Z sections representative of three independent experiments are shown. Dotted white line represents the Transwell filter that cells are seeded on. Black line = 10 µm. Confocal microscopy (60× oil immersion).</p
