<p><b>Copyright information:</b></p><p>Taken from "Transcription factor network downstream of protease activated receptors (PARs) modulating mouse bladder inflammation"</p><p>http://www.biomedcentral.com/1471-2172/8/17</p><p>BMC Immunology 2007;8():17-17.</p><p>Published online 17 Aug 2007</p><p>PMCID:PMC2000913.</p><p></p>scribed in methods. Mice were euthanized 24 hours after the last instillation; the urinary bladders were removed rapidly, frozen, and shipped to Genpathway [40] for querying the chromatin for transcription of selected genes. After isolation, the chromatin was incubated with TFEB antibody to precipitate the CHIP DNA. The final CHIP DNAs were then used as templates in Q-PCR reactions, performed in triplicate, using specific primer pairs (Table 1). Results are presented as average and standard error of Transcription Binding Events Detected Per 1000 Cells. Asterisks indicate a statistical significant difference (p < 0.05) between a specific gene and the un-transcribed region used as control and a plus sign indicates a statistical significant difference (p < 0.05) between CHIPs isolated from PAR2-AP- and control peptide-treated bladders
