<p><b>Copyright information:</b></p><p>Taken from "Peripheral infusion of rat bone marrow derived endothelial progenitor cells leads to homing in acute lung injury"</p><p>http://respiratory-research.com/content/8/1/50</p><p>Respiratory Research 2007;8(1):50-50.</p><p>Published online 9 Jul 2007</p><p>PMCID:PMC2000890.</p><p></p>-2 containing bFGF (Figure 3B). Figure 3A shows the differential growth capacities of EPC and mature rPAEC in the presence of EBM-2 supplemented with 20% FCS. Figure 3B shows the proliferative kinetics of EPC cultured in EBM-2 supplemented with 0.5% FCS towards increasing concentrations of bFGF (100 ng/ml to 100 μg/ml). Cells were seeded in 96-well plates, grown for 24 h in culture medium, washed twice with HEPES/EDTA and treated with bFGF in EBM-2 medium containing 0.5% FCS for 48 h as indicated. Cells were incubated with MTT solution, lysed and the absorbance was read. MTT activity is expressed as Optical Density and represents mean ± SEM of five independent experiments, ** < 0.01, * < 0.05
